{"id":6460,"date":"2024-01-26T17:32:19","date_gmt":"2024-01-26T08:32:19","guid":{"rendered":"https:\/\/rdec.co.jp\/update\/?post_type=products_en&p=6460"},"modified":"2024-01-30T09:55:43","modified_gmt":"2024-01-30T00:55:43","slug":"spr","status":"publish","type":"products_en","link":"https:\/\/rdec.co.jp\/update\/products_en\/6460","title":{"rendered":"SPR"},"content":{"rendered":"\n

Surface Plasmon Resonance<\/strong><\/p>\n\n\n\n

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Product Information<\/h4>\n\n\n\n

The Surface Plasmon Resonance (SPR) Spectrometer, developed in collaboration with Prof.
Wolfgang Knoll with Max Planck Institute for Polymer Research (MPI-P) in Germany, allows
users to perform a highly sensitive non-label analysis for the interaction of organic molecules
(biological molecules etc.) on a surface in real time by utilizing the surface plasmon resonance
phenomena. We would sincerely appreciate your consideration of our SPR system for your
research uses in the cutting-edge nanotechnology field.<\/p>\n\n\n\n

Principle Use<\/h5>\n\n\n\n

Direct measurement of interaction between DNA\/RNA, protein(antigen-antibody), sugar and
cells Analysis of adsorption rate and determination of bonding amount and coupling constant.<\/p>\n\n\n\n

Features<\/h5>\n\n\n\n
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  • Label free (no need to use fluorescent dye labeling)<\/li>\n\n\n\n
  • Real-time kinetic analysis of adsorption and desorption phenomena<\/li>\n\n\n\n
  • Flexible system configuration allowing users to customize attachments(electrochemical cell, CCD camera, temperature control, etc.) to meet the research usage.<\/li>\n\n\n\n
  • Measurement software using LabViewTM<\/li>\n<\/ul>\n\n\n\n
    Surface Plasmon Resonance: Optical Principle<\/h5>\n\n\n\n

    When the incoming light is reflected on the interface of about 50nm thick metal layer through a prism,
    at a certain angle of incidence in total internal reflection, the characteristic light absorption (attenuation
    of reflected light) can be observed. This is the Surface Plasmon Resonance (SPR) phenomena. It is very
    sensitive to the change of reflective index of the media, i.e. molecular adsorption to the metal interface in
    the evanescent light distance, and monitoring the change allows high-sensitivity measurement of
    molecular-adsorption movement on surface.
    The irradiated light on the backside is totally reflected and generates the weak energy wave
    (evanescent wave) on the metal layer side, then the interaction between the materials induced on
    the sensor-chip surface differ the dielectric constant and influence the surface plasmon. Thus, we can
    recognize the interaction between the materials as a change of resonance.<\/p>\n\n\n\n

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    Measurement Modes<\/h5>\n\n\n\n
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    Angle Scan Mode<\/h6>\n\n\n\n

    The system sweeps the angle of incidence and monitors
    the reflected intensity with \u03b8-2\u03b8goniometer.
    Angle scan mode allows users to determinate the
    adsorption quantity of molecules.<\/p>\n\n\n\n

    Kinetics Mode<\/h6>\n\n\n\n

    The system fixes the angle of incidence and measures
    the reflected light intensity as a function of time.
    Kinetics mode allows users to monitor the
    adsorption\/desorption process of molecules in real time<\/p>\n<\/div>\n\n\n\n

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    Configuration<\/h5>\n\n\n\n